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11. 6. 2021, 13:22
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Lab Technician Wanted!        Laboratories of Dr. Konstantinos Tripsianes (CEITEC MU) and Prof. Vítězslav Bryja (Faculty of Science MU) are looking for a lab technician/researcher with a background in biochemistry / protein purification / molecular biology (with M. Sc. / Mgr. title). Proficient communication in written and spoken English is needed. Workload: 40 hours per weekStart date: as soon as possible / upon agreement
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Work Tasks        
General molecular biology tasks: DNA and protein analysis, transformation, DNA extraction, cloning, PCRGeneral cell culture tasks: mammalian and insect cell culture, transfection and transductionProtein expression: bacterial, insect and mammalian systemsProtein purification: AKTA-based purification methods (affinity, ion exchange, size exclusion, etc.)Other tasks: preparation of buffers, SDS-PAGE, western blotting, protein quantification The list of experimental tasks is indicative – the proficiency in the methods is an advantage but the person will be trained by the members of Tripsianes/Bryja labs. |  |  |  
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Information about the laboratories        Groupleaders: Konstantinos Tripsianes, Ph. D., CEITEC, University Campus Bohunice, building E35Prof. Vítězslav Bryja, Ph. D., Department of Experimental Biology - OFIŽ, Faculty of Science, University Campus Bohunice, building D36
 Main objective of the project: Biochemical and structural characterization of Dishevelled and its phosphorylation |  |  |  
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